The embryo pooling method, also called ”pooling", is one of the treatment options that can be included in the process to increase the success rate of IVF treatments.
Under normal conditions, the eggs harvested from the woman and sperm harvested from man are fertilized in a laboratory environment using various different techniques. The development of the formed embryos is monitored and they are then transfered into the uterus. In the pooling method, the resulting embryos are frozen. The transfer into the uterus is not started until the targeted number of quality embryos is obtained.
If the pooling method is included in the treatment process, eggs are harvested multiple times before embryo transfer occurs and the quality embryos inseminated from the harvested eggs are frozen and accumulated.
When at least two high-quality embryos are obtained, the frozen embryos are thawed together under controlled conditions. The simultaneous thawing of frozen embryos facilitates the evaluation of embryos. Thus, it would be possible to choose the highest quality and genetically healthy one among them.
The embryo pooling method is often preferred because it allows obtaining a sufficient number of high-quality embryos and reduces the stress level of couples in case of repeated IVF treatment failures.
Embryo pooling in IVF treatment can be utilized if;
If preimplantation genetic diagnosis is recommended, it is possible to resort to pooling method to increase the number of embryos. The embryo pooling method reduces PGD (preimplantation genetic diagnosis) costs.
The pooling method is considered as an intermediate treatment option during the IVF treatment process. It does not change the basic stages of IVF. First, female patients are treated with ovarian stimulant medication. Maturing eggs are harvested. The harvested eggs and sperm cells are brought together and if fertilization occurs, the embryos are frozen in a way that maintains their viability, usually on the fifth day. All these stages are repeated again in the next menstrual period. After the procedure, which continues in a minimum of two cycles, when the sufficient number of embryos are harvested, the thawed embryos are transfered into the uterus.
The endometrium is monitored before embryo transfer is performed. If it is not of the appropriate thickness, treatment options such as endometrial PRP and endometrial scratching may be included in the treatment process in order to increase the success of transfer.
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